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CRIMIC CR02516

Gene Cpr47Ea

CG9079

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2R:11251751..11253398	47E3	FBti0214966	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCATCACCTGTAAGTGCAGGGAA	TCTGCTGCCTCAGTTTCATTCAGG

Images

Construct Design Sequences

sgRNA sequence	TTCATATGTGAGAAGCTCTTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCGACCTGAATCCCGATGGCTCCTATCAGTACAAGTAAGTTGGATCCAGGGATGGTAGGATCAATTTCAGGATTTGGAATGGATAAAAACAAATCGAACAATCTGAAATTAAGTTCAATTAGGATCATATAGAGAATCCTACAAAACATTGCATTTTAATAGCTTTAAAATTTTTTAATATCTTCATATGTGAGAAGCT	CTTTGGAATCAAAAAAAATCAAGGATTTTAAATTTAAATCCATGTGTTTTTTTTTCTTTTTTAAGTGTTTCTAATGAGTATTATTATAAATTTTTAAAACTTGTAGCTACGAGACAAGCAACGGAATCCGAGCGGATGAGGCTGGCTATTTGAAGAACCCGGGCAGTCAGATCGAGGCTCAGGTTAGTGTGCTTTTCAGT

 

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