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CRIMIC CR02517

Gene SmydA-3

CG43129

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:10850141..10854604	32B1	FBti0216948	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGTGCAATGGACCGGTGGTGT	TGCATGATCAGCTCCTCGCTGAAT

Images

Construct Design Sequences

sgRNA sequence	TATATAACTTTCATCTTGAGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AACAAGCGGATAACGCCCATTTCCGCCTGGAGTGCAGCCAGCTGAAGGATGCTCGGGCACGATTCTTCCGCCTGCCCAGCGGCAGCAGGCACTGTCCGCAATTGGACTGTATTATGCCGCTCAGGTGAAGCCCAGCGGGTGGAACAAAAATAGACCACCAGGCACCAGGCAAACTGATAGCGTCACTATTTTATCCGCTC	AAGATGAAAGTTATATACAAAAGGATTCATTAACAATTAAAAAAGAGAAAGATTTTTAATTTAATGTAGCTAATTATAATCAATAGGTTACTTGTTGTGCTTCATATGATTGTCATTTGGAATACCCTTTTAAACTTTCTACTTTCAGAGTTCTGTTGGCCAAGGAGGCGAATCCGGAGCGATGGGACAACGAGGTGGCA

 

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