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CRIMIC CR02521

Gene Vajk2

CG16885

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	2L:13932559..13934692	34E4	FBti0216950	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTCTCCTGCTTCTTCTCGG	GCACGACTTCATCAGCTCTTTC

Images

Construct Design Sequences

sgRNA sequence	CGCCGCATGCCCAATATGTCAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCATTTTAACCCGAAAAGGATAGGCAGTCCTAGAATATGTTTTTAGCATTTAAAAAAGAAAACAAAAATGTAGATTCGTATTAAAAAGCTTTGAACGCGAAACTACAAAGAGTTGAATTTGCCTTCTTCCAATTTGTATTTTAACCGAATTATTGCGAAAATATCAAGCCACATTAAGTATACGCCGCATGCCCAATAT	GTCAGGGGGCCTAGGCCACAATTGAACTTGGCTGGTTGCCCAGCCAACTTTCCACCCAACATTGCATAATTGTCATATTCCAAATTATGGAGTAATCGGGCGGAGGTGGAGCCGGAAGCGGCTGCAGTTGCATAACCGCCCGACCAAGCCAATTGGGTTTCTATCGAATTTAACCCACTTGTCATTGCAGCTCATTTTCG

 

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