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CRIMIC CR02529

Gene Dop1R2

CG18741

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:29630304..29659984	99B5-99B6	FBti0216200	92685

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAAGTTTTCATGGCTGCCTACCA	CGTTTACCTTTGGTGGAGTAAGC

Images

Construct Design Sequences

sgRNA sequence	TTTCCCAGACCGTAAAAACGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGCCCCGATCCTAACTGGCTTCTCTTCCAGGGCCTTTGTGCGTCTGCTGTGCATGTGCTGTCCACGCAAGATTCGCCGCAAGTACCAGCCCACGATGCGTTCCAAATCGCAGGTGAGTGTTCTCCGACCCACTCCATCCACTGCCATTCTCATCCTCCGACAGGCGATGCGAGTCGATGTGTTTCGATTCTCTTCCTCGT	TTTTACGGTCTGGGAAATTGGGAAACTTGGCGGTTTTCCACATTTTCTCCTTCGCTGCTGTCTTGTTGTTGCATCCACTTTTTTGGGTTTGATGGAGACCGCTTTCGTGAATTAAGCGCTCCACTTTCGTATGCTCCAAAAACGAAACTCAACGAACGGCGAAATTAATTTACAAACTTAATGAATATGCAAAAAAATAA

 

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