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CRIMIC CR02533

Gene Ppcs

CG5629

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:19139054..19147910	91F3-91F4	FBti0216953	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTCTTATGTATTTTCTCCCCTTTAACA	GGTGAAAGCTTTCATGGTTGAGCC

Images

Construct Design Sequences

sgRNA sequence	GTCAACTGGTGTTTATTCACTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTTTTTGTTAGTTTTTAATTTAACATTTTAGAAATTTAATCTTAGTTAAGTTTCACTTTAAGTTAAGTTTTCCACTTTAAAATGCTGCTATGCGTAAATAATATATATTGCAAATATCTGCAAGTCTTGCAAATACTTGATTCGCCTCCTAAAGCTCAAAGTTAAATATGAAATTATGTGGGTCAACTGGTGTTTATT	CACTGGGGCCAGCTTAATCCGAACATGATTTACGCAAATAGTTAACAACGAGGCACATTAGTTTCTCTCTATAAGATTTATGGTCAACTTGCTGATTTATCTGCCTGATTTGAATAACTAAATGCTGCCAAATATCTTGGATTTGTTCTAAGTCATTGGATTTTACATGAAAGGGACTTGGTTATTCTAATTTGTTTTAT

 

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