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CRIMIC CR02534

Gene fau

CG45077

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:10773197..10777374	86C6	FBti0219141	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACTGACCGTTTGTAGTTGGA	CGAAGAGAACACCAATTGACTGGG

Images

Construct Design Sequences

sgRNA sequence	GAGACGGCGCGATGGAGTCGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCCTCGATCCGATCAATCGGGCCTCCATCAAGCCCGACTACAAGCTGGCCTACGAGCCCATTGAGCCGTATGTCTCCACCCGGGACAAGAACCGGACGCGGATCCTGGGCATGGTGCGCCAGCACATCGACACCGTGGAGGCGGGTGGCAATACCGCCGGCCGCACCTTCCGCGACAGTCTGGACGCCCAGCTGCCGCGA	CTCCATCGCGCCGTCTCCGAGTCCTTGCCAGTTCGCCGGGAGACCTACCGCAACGAGAGGTCGGGCGCGATGGTCACCAAGTACTCCTACTAGGGGAGCATCATCCTATTCACCCAACCGATAACCCGAACCCAATCGAAGCCTAGTCGAACGAACCTAGTCGTATCTAAGGACACATAACCAACTAAAACAGACAAAAA

 

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