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CRIMIC CR02535

Gene Glg1

CG33214

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3L:21497549..21507538	78D4	FBti0219142	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTATGATGCGCTGGCTGCAAAGGT	GTCTTCCATTGTCAGGATCGTC

Images

Construct Design Sequences

sgRNA sequence	TTGGTAGCCAGAGTTATTACCGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATCTGAGTGTCCTGCCCCTCCAGGGGATGCGTGGCGAAGAGGTAGATGTTGTCGTTCGAGTGATGCACTCCTCCATTGAAGGGATAGTGCGAGTTGCCGAACTCATATCCAAATCGAGTGTATGTTCCCTCGTACTCAAAACTGTAGACGTAAACTGGCGTGGACCGTTGCTGGGTGTACATTTTGGTAGCCAGAGTTAT	TACCGGGGACTTCATGAAGATGTTCGTAGTGAGCTGTAGGAAATTTACAAATTTAAACTTTCTGTTTTTGCTGAGCTTTTCTCGAACTTACATCGAAGTACGAGGACACTGCTGCACTATGATCATGACTGTTAAGCGCCTGAGGCTTAAAGAGCAACCGATTTAATATGTTATCCGTGAGTCCCGATGTGTCATTGACC

 

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