CR02538

CRIMIC CR02538

Gene cv-d

CG31150

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:15844466..15853581	89A5-89A6		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TCACACAGTATGTCCCAGTAGGTC	ATAGGTCGAGAGCACTGGGTCTTT

Images

Construct Design Sequences

sgRNA sequence	AGGTGGCACGCAGGCACGAACGG
sgRNA2 sequence

left_200bp	right_200bp
GAGTCGTATTGATCAAACGGGGCTACTTACACCGCCAGGCCTGCTTTACTCTAGTCCGGTTGCCAGACTCGGTTCTCGGTCTCGATTCCGGGACGCAAACAACCCACGAATGTGGGCCAACAATTGCGGCGACTGCTTGAGTGCTTGAAAGGATGGGTTGCGTGTGTGTGTGTGTGTGTAGTAAGGTGGCACGCAGGCAC	GAACGGTGGGGCGGAAGAGGGCCATACAATCATACAGTCAGGATGTGTGTGGGTTCGCTAATCGTTGCTGCAGTTGTTGTGGCTCCGTTGTCTGCTGTTTCGTCATGGTAATGTGGCCCACTCCACTCGCCTGGAGCTTCAGCGAACAGCTGAACCCCACATTTTCTTGCCTCCACGCTTGAGGAAAGCAGTGTAACTAC