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CRIMIC CR02547

Gene CG6420

CG6420

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:26719847..26725216	97C5	FBti0219148

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multiple weak band	OK	AATGGCAAAGCGGAACAGGCTCAA	CAGCGAGCTTATCACACCAGAGAT

Images

Construct Design Sequences

sgRNA sequence	GGATGGTCGAAAGCCGCACCAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAGTGTCCCCCCAGTGTGCTTAATTTGCTCCACTTCGAGCGCTTGATGATCAATTCGCTTGTTTGGCGTTTTTATGAATGTTTATTGCGCTCGAAGGCAGTCGGTTGTGAGCGGTTTCAGCTCATCCCTGCGCGCGGATTACCCTAACTTTTGTTTGCACCTGCTGTAGTCAACGAACATTCCGATAGAGTAATCCTGGT	GCGGCTTTCGACCATCCGAATTGCTCATCGCGTCGCTTGTGTTTGCCCTGTTTCCCCTGCCCAGCAGCTGTTTCTAATTCTTTGGCATTCTGTGCGGCAACAGCTTGCGATTCTCCTTATGTATTTGCTTTAGCTGGCTTCTTTGTTGCCATAGTTATCTGCTGGCGAGAACTTGGCAAGGCTTGCATAGAGCTTTTCCC

 

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