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CRIMIC CR02554

Gene CG5196

CG5196

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:12408316..12411768	879B9	FBti0219150

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCTTTCAGGCATCATCAAGTGC	CGACATCTATGCATACAACGGACC

Images

Construct Design Sequences

sgRNA sequence	AGGTGTCTCGAACTAGGCGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTCGCCGGATTCGCCCACCAGGCCCTCTTCCTGCTCCTTTCCACGCTGGCCACATTCAATTACGTCATGGCCACTTTGACTGGTCCCGGACTGATGCCAAAACAGTGGCATCCCAAGGTAACCGGAGAACCAGAAGCGCACGGCACACGGAATGGTGGTGAAACATTTGTTGTGACACGATGGCCGGTCAACCGCCTCCG	CCTAGTTCGAGACACCTGTTACCTGTTTCCATTGATAGTGCCCACCATTTGGCCCGAAAAACTGTTGACCCGTCACGAACTGCCCAAGTACTTAGTCCGGGTGTGGTTTCTCCGTTTCTTTGTGCATCGTCATTCCACGGCGGAAGCAATTGTTTAGCGTCCGATGAGCAGGTGCCGAGTTTTCCGGAGGAGTTCGCTCT

 

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