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CRIMIC CR02556

Gene Karybeta3

CG1059

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:4649176..4654624	82C5-82D5		94459

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACAAACGTCGGTGTGCGAATCCT	TTCCACCTTGACAACATTGGGCTC

Images

Construct Design Sequences

sgRNA sequence	GGTGCGAAATCCATGACACACGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGCTTTGCCCCGACATTCGCATGTGTGCGAGGGAGAGCCGACGGACAGACGGTGCGAAGCAAGCAAACAAAGCAAAATGGAAGCGAATTGTGCAATTTGCATGGCCGCCACATGATTTTCCATTAGAATTCGTAGTAGGCAATTCGGTTTACCGATTGCCGCCGCAAACGGAAACAAAGAGCAGGTGCGAAATCCATGAC	ACACGGTTCCAGAAATGCAATCTGTCGGTTGATAAGCCACTCGGATTCCGAAGTCTCGCTCCGGCGCCACATGGCACCGCCACGCCCACGCCACACCAGCCAGAAAACCCCACCCACTCTGTTGACCTTGGGCAGATGGCAAGCATGGTACCGCTTCCACCCAAAGTTCCACCTGGATTGCTAAAAATACCTATTCAGAA

 

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