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CRIMIC CR02560

Gene Ada2b

CG9638

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:8236632..8240515	84F5		94461

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACCTTTCTGAGATTCGGTTGTGG	GCAAGACCGTAAGTTTAAGGCTCC

Images

Construct Design Sequences

sgRNA sequence	AGTTTCGATTTCATTCCCCTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TGTTTCACAAAAATTAAAAGAGGTGTTAGGTGACCGGAACCGGTAGCTTTATAATTTTATCAACAATGAGTAGATTAACTTAAATTGAAAGAAAACAAATGATTTAAGTTGAATGTATAGATAAAAGTTTGGATAAATCGATTTGGAATACTTAATACAGAATATTAAATGGTCAAATTGGCTAGTTTCGATTTCATTCC	CCTTGGAGTAAGCTTTTCCCACTGAGAATGCATGACGAGTTGAATATCGTGTGTTCTGCCTTGCTTTAACCGTCGTCCTTACCTGAACTGACCCAACTCTTACAATCGCTTTTCCGTCTTGTCTCACGATATTCAGCTCCACTGCATAATAAACGCGCCAAACAACGAGCGTTCGAATGGTTCTATAAGACAATGTTTAA

 

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