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CRIMIC CR02562

Gene Nelf-A

CG5874

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:21223704..21229512	93C7		94462

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCAAACGCAGGTCAAACTGAAG	ATACTTGGTCTACCAGCTCAGCAG

Images

Construct Design Sequences

sgRNA sequence	CCAACCCGCGAAATCGCCCGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AATGGCGTTGGTGTATGGAAAAAGTACACCAGTGCATACGCACGCATACTGCGGCCCGCGCGCACACGTACACGGAGTACACTTACCGTTGTTGAAACAAAAAGTGCGGACAATTTTTAAATACTCGGAAAATCTCTTAGATCCCATCACCTGGCGGACCCCAAAGCACATTTCTTCGCCTTTTAACATTTCCCCCCCGG	GCGATTTCGCGGGTTGGTGAAAATAAATATTAGCAACGTTTCCCGCCACTTCTGCTTTTGTGTACACATTTCTCTCTCTCCGACACTTGTGCGTGGATTTCTCTCCCGTGTGCCTCGGTGTGTTCATGTGTGAGTGGGCTGTTGTGGGTGGCCGCGGATTCATTTGTTTGTTGTGCCCTTCCCACAGAGCAATTCGAATT

 

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