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CRIMIC CR02573

Gene unc-13

CG2999

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	4:867824..912331		FBti0216954	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTCGACGCTATCAAGCTTGGGTT	CCTAGGTACAAGAGCTATCTAGCC

Images

Construct Design Sequences

sgRNA sequence	CTGAGTAACGGTGATATGCTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATATCACCGTTACTCAGTTAGCGGGAGTGCAACTAGAAATTTCAAAATATATATGTTGGGTATAGAATCTACAATATCTACAAGATTTTTACAACTTTGAATATTGTGGTGGTTTTTTGTAATATGTAAATGTAATATCTTGTAATATAAGATATAATATAGATATATCCCTCACTTTTTTATTTATTGAACAACTTCTT	ATATCACCGTTACTCAGTTAGCGGGAGTGCAACTAGAAATTTCAAAATATATATGTTGGGTATAGAATCTACAATATCTACAAGATTTTTACAACTTTGAATATTGTGGTGGTTTTTTGTAATATGTAAATGTAATATCTTGTAATATAAGATATAATATAGATATATCCCTCACTTTTTTATTTATTGAACAACTTCTT

 

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