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CRIMIC CR02574

Gene Arl4

CG2219

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	4:177348..179389		FBti0216955	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TACGATGGATGCCACTGCATGTAC	CCCTGCGTAAAAGAACAAACCTGC

Images

Construct Design Sequences

sgRNA sequence	GGCTCTATGTAAGAAGTGGGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACAACTTATTTATTTATTAACGTAAAAAAATGAAAGGAGTGTTGTTGGACATTTTTAGCTTAATAATATTACAAAAACCAAAAAAACTTCCATATGGTTTTCATCAAAAGTCATTATACACCTCATTTTGTTGACTTCATTAAGTCGGCTTGGCTTGGAAGAAACTAAAGTTACCAAATAGGATTGGTCCACTTCCACCC	ACTTCTTACATAGAGCCGCTTCTAAATGTAATTTGTCGCCTATTTCCTTCCACCATTAAGTTATGCTCCAATGTATTCCACAAGTGTTCATTAAAGCCGTGTTCTTTGGGTCATTGTCCTGATAAAAGTAAAATGAAACAATTCCAAAACGATCTGTGCTCTGCTTCAGATTTATCTTTAGGATGTAAATGTACATTTTG

 

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