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CRIMIC CR02575

Gene su(f)

CG17170

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:23096771..23101431	20E	FBti0216956	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGCTGGTTGACCGTTACAAA	TTGCCTCGCTATCTGTTTCGACCT

Images

Construct Design Sequences

sgRNA sequence	GACAGTTTGTAGGAACTCATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTATTTATAAAAATATAAGAAGAAAGAAGAAACAAACTAGCATCCATAACCGCGAAATAATTGGATTCTATTGTTATACTACATCAAACGATAACATGTGATCGTGTAGTAATCGCCATAACAGTTTAATGTCTGAATACATTTTTTGAAAATTGATTTCATTCATAATTTTTCCCATTTGAGACAGTTTGTAGGAACT	CATTGGAAAGGAAAGAGCAAGACTATTTTATTTTATATGTGCGAAGAAGAAAGAGATAGCATATAGACTTACTGAATTTTAAAAAACATTTTTTAGCACAGACTGTTTTAAAAGTGTTCATTACGACATTTTTCTTCTTATAGCCAATCAAACTTTTCACAATACTTTTTATAGGGTCCACAAAATGTTTGTCTTTAATT

 

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