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CRIMIC CR02586

Gene rho-4

CG1697

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:11599838..11606288	31D10-31D11	FBti0219156

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTGATCCGTTAGCTGGAGCAGA	GGGTACATATCAGCGCATTCATCC

Images

Construct Design Sequences

sgRNA sequence	GAGCCGCTGCAGGAATCGTCCGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGTCCAGTTTGGCAGCAGAGTTAACCCCGTTGCCCTGCCCTGCCGCTCCAGACGCTGCAACTGGCGGCGCTCCATGGCCATCAGTTCCCGCTTGGCCCGGGCCCGCACCTGGTCGTCGCTGGTCGTGGTGCAGAGACTCAGGGCCGCAAAGATGCCGGCCATGCCCAGGTAGAGAACCAGTACGGTCACAATGCCGGAC	GATTCCTGCAGCGGCTCCTCCTCCTCCGCGACGTCGCATGTGGGCGTCGGTGTCCAGCCAAGCCAAGAGGTCACGTGGCGGGTCATCTCCATCAGTTGCGACATCGTTCTCGGTTGAATCTTGAGGCGAAGATACAAATATATATAATATATGTATGGTTTATACTAAATAATTATGTCATTAACCGATGACACCAAGCA

 

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