CR02595

CRIMIC CR02595

Gene SmydA-9

CG12119

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:9206126..9210887	8D1		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AGTTCCGATACGCAGACTGCACTA	AGAGCGATTCGGATCGTAATTCCC

Images

Construct Design Sequences

sgRNA sequence	CACACGTGCCACACACTCAAGGG
sgRNA2 sequence

left_200bp	right_200bp
CTTGGAATCAGATTAATATAACCATAAAGAGGCTATAGTAGAAGTCGTTTCGAAGATGATGTCTAGAAATAGTTTAGTATTCAAGCATTTAAGCGTTTGCTATGGAAACATCCTTCCTTAAATCATCACTTGTTGCCAATATAATAGTTCACTAATTTATTGAAAATATTCAGACTGTTGCTGCTAATCGCCTGCCCTTG	AGTGTGTGGCACGTGTGTTGGTATAACTATAAAACATATAATCTCAACTATGGTTCAGCGAACTCCCGCAAATGGAATTTATTTATATAGTATATAGTGGTATATGGCTTCCTTCGATCCGAGTGCTCATCATTCGGCATCCGTTTCGTTCTTTATCCCGATTACAAGTTTATTGTTCCAAACACAAAATATTGAGTTTC