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CRIMIC CR02602

Gene Evi5

CG11727

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:11430523..11454386	10B11-10B14	FBti0219162

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCTTACAGCAAATGGGTTACGG	GCGAATCTGGATGATTCGGACAAG

Images

Construct Design Sequences

sgRNA sequence	TTGGAAACGTGTCTCTGATCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCGACATGAAGGCGGAGGTGAGCGTGAGATGATCTGCAATGTTCACAGCATTTGTCGCTAATCCCCCACTGCTTTTGGTTTTGGTTTCGGTTTCGGTTTTAAGTTCATTCAAATGCCAGCAGCAGCAGACATTCAATAATAACACGTCCACCCACTCATTTTATGTCCACCCATCCAACTGACTAAACCAGAGGCCAGAT	CAGAGACACGTTTCCAATCTGGAGGAGCACGCTGTGGCCTCCTGTAGCGGATTTTCACTATAAATATGCTTTGTTTGCTAGCTGAGCGCAAGTCAAATCTGTTTGCTATGCTCACATTGCAAAATATATCGATTTGACTTGAAGTTACTAATTGAAAAAAGAAACCAAAGAATTTTTGAGTTTGCTGTGCTTTACTGTAC

 

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