CR02605

CRIMIC CR02605

Gene Nup153

CG4453

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:16599241..16610459	14F2	FBti0219164	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	AGCTGCTGCTCCATTGACCAACAT	TCAACAGTAACAACACGCACGACC

Images

Construct Design Sequences

sgRNA sequence	ACATTTGTATGGATGAAACCTGG
sgRNA2 sequence

left_200bp	right_200bp
AATAATCCCTCCGGTGGCGATGTAAGTATACGATTTCCACCCAACTCCCAGTAGATCACCAAACCACCAGTCCACCACCCCGCCCCACCTGATCAACTTATATAGTATTCGCAGTACAAGCACAAGGTGATCGATGGGAGATGAGCGCCTGATGTGATGATGAAGTAGATCGATCACACCACACACTCACTCACCCAGGT	TTCATCCATACAAATGTGTTTGCGATTGTTTGTAGTCGTAGCTGTCCGTCATATTTCCTTTTAATTTTCCGACTTGCATGCCCACGTTATAGAAATTATAACAATGCGATCAAAAATCGATCGATCCCCCTAATATAAAACATACCTGGCCGACATTGTATATTAATTTAGAGCTAGTTACTTACCGTATTGATTTTACC