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CRIMIC CR02616

Gene Kap3

CG11759

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:11334187..11340084	10B3	FBti0217653	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTAGTTTAGGACCACTTCAGGG	TTACACATCGCTGCACTGGGAGAA

Images

Construct Design Sequences

sgRNA sequence	TTGTTGTTGCGCTATCGATTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTTGATCCTTAATCGAATAGGTTGAAAAATTGATCTAGATGTGTACACATACATATATTGCTTATTAGTTTAAATACTAGCAGTCATTGTTCTACAAAAACATCCGAATGAGTACTTAAAAAAGCTACTTCTGTTTCTTCCGGTGTGCCCCGAAAAGGGTTAAGGGGCGGGGGGCAGAAGCTGACATTGAGCGGCCCAAT	CGATAGCGCAACAACAACGGGGTCGATGACGGCCTGCGCTCCAAGTAGTGAGTGAAAAATGTAAAACCACAGCAACAAAAAAAAAAAGATACCCAAAAGCGGTTGGTTGGCCGGGTCTTTAACCTCAATTCACGTTTTTTGGCCGAGCGACAAGCTTATTTTTCGCCGATTGTTCACGACCATAAATATGTACTACATAA

 

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