CR02617

CRIMIC CR02617

Gene Ir8a

CG32704

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:9232726..9236585	8D2	FBti0217654	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGCACGAGGATTCAATGTGAACC	CATTGGCTATGTGCGTTTGGAG

Images

Construct Design Sequences

sgRNA sequence	GCTCTTCTAAGACGGAAAATCGG
sgRNA2 sequence

left_200bp	right_200bp
CTAGTCGGCTATCCCTTTCGGACACTAATCATGGACGCCAGTCAGACCGATCCTGGCCAGCATTTCCTCGAGCGCATTCGATCGCTGCGTCCGGCGCCAACTTATATAGCCCTATTCGCACGCGCTGCCGCCATGAATGGCATTTTCGAAAAGGTGAGCCCAGTGGTCAAAAACCGTACGGAAATGGGAATTTTCCGATT	TTCCGTCTTAGAAGAGCACTTTTCTTGACCAAAACATTAGATAAAATGATCCAAATATTAAGTGTTATACCTTGTTGAGCTCGTAATTAAATTTCCAATCAAATTGAGTTCAAAAAATGACATTCTATTTTTTGTCAATTTTTTGCAAATTTTGATGATGTTACCCCTTACAATAAATGCGAAAATTTGGCCAAAAATTA