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CRIMIC CR02617

Gene Ir8a

CG32704

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:9232726..9236585	8D2	FBti0217654

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGCACGAGGATTCAATGTGAACC	CATTGGCTATGTGCGTTTGGAG

Images

Construct Design Sequences

sgRNA sequence	GCTCTTCTAAGACGGAAAATCGG
sgRNA2 sequence

 

left_200bp	right_200bp
CTAGTCGGCTATCCCTTTCGGACACTAATCATGGACGCCAGTCAGACCGATCCTGGCCAGCATTTCCTCGAGCGCATTCGATCGCTGCGTCCGGCGCCAACTTATATAGCCCTATTCGCACGCGCTGCCGCCATGAATGGCATTTTCGAAAAGGTGAGCCCAGTGGTCAAAAACCGTACGGAAATGGGAATTTTCCGATT	TTCCGTCTTAGAAGAGCACTTTTCTTGACCAAAACATTAGATAAAATGATCCAAATATTAAGTGTTATACCTTGTTGAGCTCGTAATTAAATTTCCAATCAAATTGAGTTCAAAAAATGACATTCTATTTTTTGTCAATTTTTTGCAAATTTTGATGATGTTACCCCTTACAATAAATGCGAAAATTTGGCCAAAAATTA

 

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