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CRIMIC CR02630

Gene Mtpalpha

CG4389

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:9573563..9576769	30B12		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTGGAGATGCTTGTTGGCAAC	GGTGGATGAGTTTGTACTGTGTGC

Images

Construct Design Sequences

sgRNA sequence	AGGCCATAATTCTTCTACATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTAAAACAAATAGTTATCGTAAGCAAAAAGTGACTATCTTATAAGATCGAACTGAGAGCAATGTCGGCAACGAGGTTTTTGAGTGCCGTTGGCCAAATTTCGCGACAGCAATTGCTGCAGAATAAGTGCACCAGAGGTGGGTTAATTAGAAGTTCTGCTATGAAAAGGGAAAGTGCAAATTGCAGGCCATAATTCTTCTA	CATTGGAATTTGGCCCCTTGGAAATGGGCAGTACTCTAGCGGTATCTCTGTCTCTCTCCCGTTCCCGTGTTAGCTGAATCTCCCCCGATGCCGCATAACTGGACTTTGAACTTTGTACTCTCCCTTGACGTATAGCTTCTGTCTCTCGCTTTCTGATTTCCGATTTCGAGTGCCGTGAGATAATGTGCACTAATCGCAAA

 

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