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CRIMIC CR02631

Gene zf30C

CG3998

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	2L:9758342..9762375	30C7	FBti0219168	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGATAGTTGGTTCGAAAGGAGAC	TCATCCTTTGCATGCTGGGTGA

Images

Construct Design Sequences

sgRNA sequence	TGTATGCGCCTACCAGGGCTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAGTAACCAAAAGAATAATAATAAAATTTGATAGTTTGCTTAAAACGAAAAAATAGAAAAAACGGAAGTCACTAGATCTAATTTGATTTACATAGTTATTATTATTGTGCACAATGCAGCTTCTTTACTAAACCCTATGATAAATATAAATACAATAATATTGCGACAAGTGCATTTAAGACAGAACTGAAAGACCCAGC	CCTGGTAGGCGCATACAAACCCAAACCAACCGAAAATCAAATTTGCATTTGTTTACGTTCGCCCCAACATGCTATAATCTCAATTCCCCACTCGCAGAAACCGAGTCGATGGCTCCAAGCAGCAGTGCGGCGGCTGCAGCCACCAAGCTCCTGGTGGAGATCACCACCGACGGAATACCCAAGCTGCACTGCCCGGTGTG

 

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