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CRIMIC CR02634

Gene nAChRbeta1

CG11348

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3L:4430811..4430812		FBti0228080	97550

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTCGACAACTGTAAACTTCTGTACCC	GGTTCTGTCTATGCGAACTTCGAC

Images

Construct Design Sequences

sgRNA sequence	CCCATCCTGCGGGTCCCTCAAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTTTACAAACAATATATAATATTCCCCAGCTATAATCGCTTTTGTAATTATTAACATTTCTCCAGAGTCCCCTCTGCATTTCTCTCGCTCGCTCTCTCTCTATGACTCTCTATCTCTCTGTCTTTGTCCACCCCCCAAAATAAATATTAAAAACCCCTGACTGTGTATCTACGAAAAGCGTGGTAGCCAAGGTCCTTGA	GGGACCCGCAGGATGGGTGGTGGCACATTACATCCCGAACGACCACGGCAACCGAAATGAACAGCAACCAAAAGAAACAGAAAATGCAATACGAAAATGCAACGAACCGAAACAACAATGCATATATATATATACGTGTATATAAAGATTTCTGTATCAACTGCCGAATCTTAATTCATACTCCTTCTCTTCTGAGCAGT

 

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