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CRIMIC CR02636

Gene Neu2

CG7204

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3L:21487200..21487201	78D3-78D4	FBti0228081	97551

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGAGCTGACACGGTGATATGATT	CGGTTCTCAGTCCATCCTTTGT

Images

Construct Design Sequences

sgRNA sequence	GGTCTATTCGCCGAACCCGATGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTGGGATAGTCGACAATTTAAAGATTCCTCTGTAATTACTTGCATCCAGTTTGCTACCTTTTTTATGAAGAGGAATCACAAAGAACTCCTTCCATATATAAGGGAATTGTGAAGATTCCAGAGATAAGGTAAACAGTTTCAGTAGAGGCTTGCACAAGGCTTCCGCACAGAATCTGAGCACACAGACAGGGATTCCATCG	GGTTCGGCGAATAGACCGGTTAAACACGCTGAAGATCGTTAAGCAGTGAGCTTTCGTTTATAGTGGGGCAAAATATTAGATTCGACTTAGATACCGCATAAGAGTATGGCTGTTCGGAATGAGATAAAGTTGAATATGTTGTTTGAAAAAACTTGGCGAATAGATCGGCTATTGCCTGATCCGATGTTACCGTAGTGTTT

 

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