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CRIMIC CR02637

Gene Lrrk

CG5483

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:20635480..20635480	92F4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTAAGAGGTTACACTGTTTGTTG	CAGTAAGAGGTTACACTGTTTGTTG

Images

Construct Design Sequences

sgRNA sequence	GACTATGCAAATTACCTAAGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCGGACCATAACAACTACCCATTTAACACATACCATGCATGACTTGGATATCTCGTGAAGTACACAAACCAAAACAATGAGCGACTCCGACGAAGGTGAGATAACACAACTTTTAGTGAAATTATGCACAATATGCTAGTTTTGCGCAAATTTAGTCAGAGTGCAAATGAGCATTATACGCTTGACTATGCAAATTACCT	AAGTGGTGCAATTAAAACTTCAACCAGTTGTTATTGGTTTTTCAACAACACCAGCAAGGGCACAGGGTGACATTGGTGCAATGGAAGTGGGCGCGCAAAAACTTTTTTCAAACAGCCACCCCCCACAGAAGCAACTGGTGCAACCACTGGACCATCCGACACCCACGAAGAGACAAATCAAAACAAATGCCACGCACTCA

 

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