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CRIMIC CR02639

Gene thw

CG14608

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:7235232..7235233		FBti0228082	97552

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTGGAGCTGATCTCTCTGTGACTC	GCACAAGATTGTGGGAGACTCCAA

Images

Construct Design Sequences

sgRNA sequence	GCAACGAGTTCCGTGTACCACGG
sgRNA2 sequence

 

left_200bp	right_200bp
AGCGAAGTGAATGTCTTGGGGAACTGACTGACTGTCTCACTGCCTCACTGACTGACTGACTGACTGCCTGAATGACTGACTGACTAACTGAATAACTCCTGGCCGGCGCCTGACATCTGTTCCTGGGCAGCCGCAAGCCCAATCGGATTTTGGCTGGGTTAGCCTAAAATGTAATAATTTATTGCGCATACACCCCGTGG	TACACGGAACTCGTTGCGAATCTGCATATTCAACTCTCTGCTCCTCTCAAAATATCTACGAGTATATTTGGCGACCAGATATATCTAACGATCTGCCGTAAATTTCTGCATGCGTTTGTGGGCGTCTACCGATTATGCAATCGCTGAGAGCCTTCTGCGCTTTGTTTGTTTTCTTCGGCGCATTGACAACTCTCCTCCGC

 

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