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CRIMIC CR02641

Gene Leash

CG4674

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:10890517..10890518	86C7	FBti0228083	97553

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGATTACGGTAGCGTCCAATCACA	TTAGGCTCATGAACAGCACCAGGA

Images

Construct Design Sequences

sgRNA sequence	CCTCCCTTGAGTGGCTCCGCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GGGCTGCGCGAACGGGAGTAGGTACGTCGGCGACGCGACCGTGATCGGCTAATCGATCTGGACCTCGAGCCAGAACGCCGGCTCCTGAGCGACGAACGCGTGCTCCTTCCAAGCCGACGATTACGTGAGCGACTTCGCGAGCGGCTTCTACTCCTACTCCGGCTGCGGGAGCTGCTGCTCCGTCCTCCCTTGAGTGGCTC	CGCTGGCGCTGCTAGACGCAGCATTCCCAGCTTGTCCATCTGCAGCGGTCGTTTCTTAATCTGAAAGGAGAGCTTGGGTGCCGGCTCCGGATGTTTCTTTTGCCCCAGAAACTTGGGCGCTGGTGGTGGCTGGGCGCCTCCAAAGCCAACAACGGAGGCCGCATGACGCGATGTGCTGGGTCCCACATTGTCAAAGCTTG

 

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