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CRIMIC CR02644

Gene stops

CG31006

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:31167137..31167138	100B4-100B5	FBti0228084	97554

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCACCACACCCTCCATTCCAT	CCACAATGTTTGCCAAACCAAGGC

Images

Construct Design Sequences

sgRNA sequence	AGTGAAAACTGCCTGCTTATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TCAAGCTGCTGGGTAAGTGGAGTCGGGCCAACTAAATAATTTAATTTAAATGCATATGTATGCGTATGTGTGCGTAAGCGTATGCGAGTGAGTGTGCGTGTGTGTGTGGCAAGACTTTCCTGTTTGCTTTTTGGGCGTGGCTGTGTGCTTTGCATACTTTAATAAATATCATCCCATCGCATCGGATTATTACGCCCATA	AGCAGGCAGTTTTCACTTTAATCACGTGTTTCTGCTAATTGCGTTAAGAAAGTGTTGCTGATGTCGTTCTGTTGCTCTGTTGTTGCTGCATCGTAAAACTTGTAAAGTGCCTCAAAGTGTTGCAAGTTATTTGGGGCGTACGCATGTTGGCTGCCTCTAATTAAAAGGCTGCGTAAACTTTTGCGAGCAGGAAAAGTGCC

 

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