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CRIMIC CR02645

Gene wge

CG31151

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:22717595..22717596		FBti0228085	97555

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATGTAGCTCTAATTGCGTTTGCG	GCAACTGGTGGCACCTGAAAGTAA

Images

Construct Design Sequences

sgRNA sequence	TGCCTGGTTGGCGTAGTGGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACACTGTCGAATACTTGTCATTCATTTCGATTCGAGTGCGGTGGTGGGTTTCCCAATTCAGAACCCATAATGTAATCCAATTACAGGGCATCACGTACGTTTTAATCGAATTTTTGTAGAAATTATCCAGCCACAGTGTCGGAAGGGGGTGGGTTATTCGAAACGATTGGGATGTGTGATAGTTGCCTGGTTGGCGTAGT	GGTGGGCGGTTGGGTGGTTTGGGCGCACGCGGTCACCAAATGCATAGTTCAAGGACATTGCAATATTTCACGTATACATACACATCTTCGCTTACAACTTACAAGTTACACGGCAGGCCCGAAACTTTATTAAACAAACGAACGCCAAACGAATAGGTTGGCATATCTCTTCCCAGAATTCAATTTGAGAATCAATGGTT

 

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