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CRIMIC CR02654

Gene Afti

CG8538

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:14324499..14324500	88B3-88B4	FBti0228087	97557

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTGGGCATCGATTCAAGGAATA	GTTCCAATTGTAATGTCTGTACATGTG

Images

Construct Design Sequences

sgRNA sequence	GATTTACGAAACTTGTGTAGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCTCTTCGGCGACAAGTGGAACTCATCGATGCCACGTTTTGCTGCCAACCTGAGCTTTGATCCGCTGAAGCCGCTGAAACCTCAATCAACCGGCGCCGCAAGCAGCCTGGAGCCCATTTCCAATTCCACCAGCTACCAGGGTGAGTGTCCTTAGAGTCTCTGCGAGATTGCTCCACTTAGTTGCCGCAAATTAACCGCTA	CACAAGTTTCGTAAATCGAGAAATTGCCACCCTATCGCCTAACGCTCGCTTTACTTTCAAACGCTTTTCGTTGCCTATTTCCCGATCCTATTGACACACGCTTCCCGGATTGGCCTGCCGTTCGATTGGCTGCTCCTTTCTATATATTCTTATTGATTTTCGTTTGCCTTGTTTTTCCTTGTGACCACATTACTTCTAAC

 

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