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CRIMIC CR02663

Gene Capa

CG15520

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:29913695..29913696	99C6	FBti0228093	97561

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACTTCTTGAAGAGGCATGCA	TACCATTGTAATCCTCCTGGGAGG

Images

Construct Design Sequences

sgRNA sequence	CTTGAGTTACTTTGGCGGTTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACAGTGTCTTTTTATATCAAGCTCTTTAAATCAAAAAGGTGATTTCAAATCTAACCGCTGAAATAATAAAAGCCACTTAAGAAGAGTTAAATATCAAAGAAATAGTTGTGGCACTCTAAATCTATAATTCAAATTTAAAAAAGGTTTTAATCACAAAAAGCCTTAATAAAAATATTCAAGTCACTTGAGTTACTTTGGCG	GTTAGGCTACGAAAATAGCTTATTCCACGGAGACTTAAGAACAATAAGATGAGAACATTTGAATAAATTGCAATCCCCAAACACTCCATTCAGAGACGGACCACGACAAGAACCGACGAGGTGCCAACATGGGGCTCTATGCCTTCCCGCGCGTTGGTCGCAGCGATCCCAGTCTGGCCAACAGTCTGCGCGACGGATTA

 

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