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CRIMIC CR02697

Gene b6

CG3100

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	X:1686624..1690187	2B7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
multi	multi	AGTAGTGATCAGCAGCCAAGCTTC	CAAACTCACCGAGTTGTGGAAACC

Images

Construct Design Sequences

sgRNA sequence	ACTCGCTTTCATGCCAATAATGG
sgRNA2 sequence

 

left_200bp	right_200bp
CGGTTGTATAACCAACCGCCTGGCTCACCGCTTGGGCGGGTTTCCTGATCGCATCTGATCACCGATGGGGGGATTCCTCGTGGCTCGTGAGATGACTAGTTGCCTAGCGAAACGTTCTGGCCATTGAGCCATCGAGTTGTTGGTAGTAGTTGGGAACGCTGCTCCACTCAGTTTCGCAATCTGAGCCGCCGTTACCATTA	TTGGCATGAAAGCGAGTCTCAAAGCCAATCTGATCCACTTATGCATCCACATCCACATTCACACTTGCAGCTGGAAAGGAACCACGCGAGGTGCAAATCTGGGTCGCAAATGCGCAGAATTCCAGTTTCCTTTCGATGGCCATAAAAGGTCAGCAAATGTACAGGTAAGTCTAGACGCAACCATCCATCGATCAATCCGG

 

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