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CRIMIC CR02699

Gene Hk

CG43388

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	X:10242700..10242701	9B4-9B5	FBti0228099	97567

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGACTCTTTGCAGATTCTCGCA	TTGCGTGATTGTGACAGGTGGTTC

Images

Construct Design Sequences

sgRNA sequence	AAGCGATACTTGCTTGTATGTGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAAACTATTTTCCTTTACAGGTGCCGGGCTCCCATTGCGTCACTCGACTGTATGGAGGAGTTCAGTGGTACGGGCGGTCATCTTGATTTCGGTATCAAATTTCTCACTATCATATAGGCTTATCATTTATGTTGTTTACTGTCGATTGTTTCGACTTTTTCGATTGTTTTTCGATTGCCTGCCTCTGCATGCTACCACAT	ACAAGCAAGTATCGCTTGCTTCAAAATAGCCCAAGTAAATGAAACATATACCTACATATGTATAGCGTATTGTACAAAATGAGCATTCAACTAAAGCACCCACCTCCGCAATCCTTGTAGTTCAGCTGTTGGTAATTGTTGGTTTTGTTTAGCGAATTAAGGCGCAGGGCGTTGGAGTGCTCTCTATTCCATCGAATTCG

 

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