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CRIMIC CR02717

Gene mei-217

CG33935

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	X:17141544..17141545	15E5	FBti0228110	97578

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCGTGAACAAGGAATGCT	AGCATTCCTTGTTCACGAGA

Images

Construct Design Sequences

sgRNA sequence	TCTCGTGAACAAGGAATGCTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTGCGGCAGTGGCTGTATGCGAAATGCCATACAGACCAGCTCAACAGATGGTAGGTCCCATAGGCTAGGAACATAAAGAAACAACGTGCACTTTATCCAAATTGTGGCACAGCCAACAACCTCTAACTAACAACCTCTAACCTCTAGCCATACTCACAACCCTCGGAACCCTGTCTAACAAAATGTTATCTAACCCAGC	ATTCCTTGTTCACGAGATCCTGTGCCAGCTCCATTGCTAATCCATCTTCTTGATATCTTTCAGCACCCTTTTGTTCGAGCTGCTCGCGGCCCATGAGTGAATACCAAAAGCTGCGCGTCACGGAAACCTATCGTATCTTGGCTGTCTTGCCTATTTCGGCAGTTCAGACACCTCGTGTCACCAATTGCCTTCATCGCCCA

 

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