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CRIMIC CR02723

Gene TwdlE

CG14534

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	2L:7857957..7860839	28D2		94481

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAACAACCGCCTCGAGACGA	TCGTCTCGAGGCGGTTGTTG

Images

Construct Design Sequences

sgRNA sequence	CAACAACCGCCTCGAGACGAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAATTGGAGTTGGAAGCGATGTTAATTACCACGAGAGGGGAATGATATGGCTTTGCAGACCGCCAAACCGTGACTAAAACGTTACCTAAGCTCGCCGCAGCACTTGTACTCGTATATATCTCAGTACCATTTCCAAGCAAATTATCAGGCGCAGAGGGACAGGAACTCGATTACCATAGGTAGCTTTATGTAAACCCTCG	TCTCGAGGCGGTTGTTGTGACAATCGCTGTCATCGTCACAATAGTCATCATCTTCATCTGATTAGTAAAACTGACAAGCAGCTGCAGTGGGTTCAACGTTGTCGGGAGGTCACCATTTTCGGGAGGTCACCATTTATGGGCATGCCATTTGCATGCACCTCAAAGAGAAACTCCTTTCTCCCGCATCGCGGTATTTGTTC

 

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