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CRIMIC CR02726

Gene Wdr33

CG1109

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	3R:5662191..5662192	83B8	FBti0228114	97582

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACAACGTGAAGATGTACCAGGC	GTTCTCAGTCAGATGGTTTTATTGTTAGC

Images

Construct Design Sequences

sgRNA sequence	GTATTCGCAACAGCCCTAAAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CACAAGGAGGCGATTAGGGGAATAAGGTATATAGTTGTAATTGTCGAATGTATTTACCATTTTGTAGCCGATGACACCAGCCGACCAAGGCAAAACCAAAGCCAGATTCGCGTTGCTCACATAATTCCTTTAGTTTGGGTCGCTTGGGCAGTCATGGGTATTTATTGTTGCCTAATCAACTTAGTGGATCGGTTCCCTTT	AGGGCTGTTGCGAATACATATCAATAAAGCCGTCAATATAGCAACATGCCAACATATAGCTTCGCCTTATTGGCTACATAAGTTTTAACCTCGCCTCCACTAGAACAAAAAGACTCAAATATGTTGTAAGCACCGAACTCTTCTGTGATATATCAATAAAAAATGAAAAATAAAAAGACAAAGTGAAATGATGCAAATGC

 

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