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CRIMIC CR02729

Gene rdhB

CG7077

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	3R:22355591..22355592	94A5	FBti0228115	97583

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTGCTTTTTCGTCAGGTGTGGG	CGCCATGTATGCACTGCATTTAAC

Images

Construct Design Sequences

sgRNA sequence	CAAGGTATGGCGGTGCGTCGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TAAAAGAAGTTTACGCAATGTTTGCATAAAATGCACGCAAAAATGTCCCGTCAGCTGCTGACCATCATTTTCCGTAGGTGTTCGACGGCGACTTGTTTTTTGTTGCCGTTTTAAGTGCTTGAAATCCGTTGTGTTGACTTTATTTACCCACGCACACCCGCCGTTTGTGACCCCGCTTTGTCTTTATTTTCCATCCCCGA	CGCACCGCCATACCTTGGCATGTGGACGATGCTCATGCTACTCCTCCGCCTCCTCCTTCGCTCTGCTCTGGACATGTGTATGGACAACATTTGACAACAGCGTGCTTGGCCATCGGATTCCGGAAGTGCCGGTGCCCTTATTCAGCGTTTATCCGGCCACGAAGCATGCAATTACGGCCCTCTGCCAAACGGTGCGCCAG

 

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