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CRIMIC CR02746

Gene Nt5b

CG32549

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:18403802..18403803	17A10-17A11	FBti0228125	97592

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGATCAGTCGGTGCTCAATGGA	CAACGAAGGACAGAGTATAGCG

Images

Construct Design Sequences

sgRNA sequence	TATAAATAGTCAGCGATAGTTGG
sgRNA2 sequence

 

left_200bp	right_200bp
GCGAGAACTTGGTCACAGGTGAGCAGATCGCTGGAAAACACGATCCGTTGATTCCCAAAACATAGCCGCTAATCCTGATTCAGTTTCCAAAGAAATTGGAATCATTATTAACTGGATGTAACCGTCCAGATTCTCAGAAGATTTTCTTGCAAGTCATGTTCTTGTAACCAATTTCATGGTTACAAGAAAATCCGCCAACT	ATCGCTGACTATTTATACCGTCGTACTCTCTCCTTCGTTAAAGTTTCTTGATATCAAAGCAAACGTTTAGAAATTCGGAGTGCTTAGTTCCAGTTTCCAAAAGAATTGGAATCAATATTGACTAATATTGACAGAATATTTTATTGCAACTAATGTATATGTAATCAATCGCATGTTTTCAGAAACATGAGTCAACTATC

 

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