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CRIMIC CR02749

Gene chas

CG32556

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:17675167..17675168	16B12-16C1	FBti0228127	97594

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCTTAACCAATTGGAGCCTCCTC	TCATTGTGCTGGCAGGCATCTC

Images

Construct Design Sequences

sgRNA sequence	ACAAAATGGTGATTTACTGGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCGCCCAGGAGTCGCAGTCGTTCGTCATCGCCGCAGGTCGATTACCTGATGACCAGGTGGGCTATGATTCTGATATGATATCTACATACATATGTAGGTTATTTATTTGATAAGAAGAATAATTGTTTGAATCTTTTAATTTAATTCCGATGGTCTCAATTTTAAAAATATTTAACGACGGTAACAAAATGGTGATTTAC	TGGAGGAACCCAGTGTCCCTTACAGCAATGTAAAGCAAATATAATAATTATTTATAAACTCTTTGAAAATTTTTAAAGAATTTACTTTTAATAAATAACTGTGATTTTTAACATTTTTGTTAACTAAATAAGCATCCATTTGTTTTTAGTTTTTGTTTTTTAGTTTTGTATGTTTGCAAATTGTTTTAAACAGTTAATAA

 

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