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CRIMIC CR02755

Gene Pdcd4

CG10990

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:13747422..13747423	12B4-12B10	FBti0228130	97597

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGTGCACTTGTGTGTGTGTTTG	GCAATTACAATTATGCAATGCATTGCC

Images

Construct Design Sequences

sgRNA sequence	GCGGGTGAACCAAATGCAGTGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TATTTCTGCATTTAGTACCCCACTTGGTCACATATTTATAGTAGCAACAATTAAGGCTCCAGCTGGTTTTTGCCATTGTTGTTGTTTGTTTTGTTTCTAGGCCAGAATCCTTTGATTGGCCAGCTTGGTGGTGTCCCCCTTTAAATATATAAAACTAGTTTTAATTTACCTCGCTCTCCCCACCTTCTCACCACCCCACT	GCATTTGGTTCACCCGCTACTTTTGCACAGCTGTTGCAGTAAAGAGAGCAGTGGAGAGAGAGAACCTCAACGGGAGAGAGAGACTCCCAACGGGAGAGATAGAGAGAGAGAGAGCGAAAAAGGGTCAACGGTTGAATGAACTTTTGTGCAAATTCGTTGTTGTTCTTGTTATTGTTGTTACCAAATTTGCGCCAAGCATT

 

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