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CRIMIC CR02757

Gene SmydA-4

CG11160

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:11048411..11048412	10A3	FBti0228132	97599

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCATGCACGAGGGCAAGCTCTT	TGATCGATCGACCGAATTGGGCTA

Images

Construct Design Sequences

sgRNA sequence	AGGACATCGCTCAGTGAGTGGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CCGACTGCAGCGCCCTCGTGTGCGCCACCTGTCGGACGGGATCGGTGCGAGCGGTGGATCCACTGCAGCAGACCGGCGACTGGGCGTGAGTAGTGTAGCGGGCAGTCGCGATGGTGGGCAGTGATGGGCGGGTCAACGTAATCTGCCATAATCTTATGGCATCTTATCCTTTCACAAAATTTTGACCCACCCCACCCCAC	TCACTGAGCGATGTCCTTGGGCCAGATGGGCTGTACAGTAACCCTATACTTATCTTACTCCAATTAGCTGCGACCGTTGCGCCCATAAAATGGGAGCCACGGAAGTGGAGCGCCAGTTGGATCGTATCAACAACGACCTGGAGGACATTGATGTCCACGATATTCCAGGACTGGAGAACTTTCTCCTTAGGTGCGTCTGC

 

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