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CRIMIC CR02758

Gene Ldsdh1

CG2254

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:8078350..8081605	7D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAGCTGGGAGAAAACACGATGAC	ATAATAAAGTAAACCACATAATGCTATGGTTT

Images

Construct Design Sequences

sgRNA sequence	CAGTGTAGCCGCAAAAAGTGAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACGTTGGAACCACCCAAACCCGCCCATTGCCGCCCCCCTTTTTTTTTTTTTGGCTATTTCCGCATTTCCCACGAAAAACCGAAACACATTACATGACATCATTGATAAGTAAATAACAGAAATGTGTCATAAAAAATGGAATGATACAAAGAAAATTTTTCATTTAAGCGACTTGTTTTCTCCCAGTGTAGCCGCAAAAA	GTGAGGATGGCAAAGGTCATGCGATTGTCGCTGGCTGTAAGATAAGGCATATACACGCACATACTTGCCATCCAAATGACTGTGGGTCATGCAAGAGTTGGAGGGTTCCGGATTAATCCCATTTGAACTCCACTCCGCTTGATAATTATGATGATGCCCAAGATCAAGAGTCAATGTAGAGAAAGCACGCACTTGCCACA

 

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