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CRIMIC CR02760

Gene RhoGAP15B

CG4937

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:16950413..16950414	15C4	FBti0228134	97601

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACTTCCTTTGAAGGAAACAGCTGT	AGAGAGAATGAAACACACACCAAAACC

Images

Construct Design Sequences

sgRNA sequence	ATTGATCTTGAACAGACGCCGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CACTAATCATTTACATATCATTATTTGGTCACAAATATGGCCCCCAGTTTGAAAGCATCCCTTTCTACTTATGTACTTCCCTGCGGAACTCCATGTCCTTCTATTTCGAGGTACGCCTCCCCAAGATCACTTCCGCCGCCCTGTCCAACCGTGAATATAAATGGGCCCATCAAGGGATTCGTCACTATAAATAGCCCGGC	GTCTGTTCAAGATCAATGACAAGCGGAATTGTTTAAAAATAGTAGAGCGGAAAGTCTTAAGATCGTTTTTCACACCGAAATCTGAGTTTTGTGCGGTCGACAATTGTTTTCAAGATCAGTCGGTAGGGAATTGTGTATAGGAAAGGTATTGTCGGGTGCCTTAGTCTTAATTGTATAGAATTATTAAGATATCTTATAGC

 

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