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CRIMIC CR02761

Gene Klp10A

CG1453

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p2	X:11134102..11134103	10A6	FBti0228135	97602

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GCTCTCTAAACCCAATTCTCCAGC	CGATTTGCATTCAAAGTTAGCAGTTGC

Images

Construct Design Sequences

sgRNA sequence	TAATGCCATTAGTTGCGTCACGG
sgRNA2 sequence

 

left_200bp	right_200bp
GAAGAGATTTCGAAAAAGTAACTGCCTTGTGGCCCGGCATTTGTCCATTAGCAATTGTTTGGCTTCAATTGAATTAAAGCTGCTACGGCTGCTGCTGCCCCTTTCCTTTCTCCGCCAACTTCTTGCTTAGCTTCTCTTTCCGGTTTTCGCACTCGTATGTATTGTGTGGCTGTCGGGAATTGGTAATGCCATTAGTTGCG	TCACGGCAATCGCTTAAGTATAGCCCAAAAAGTATACCCCATGAGTAATATAGCCCGGCATTAATGCACAAAATCTCGCCGATGATAACGTAGTCTCGTGTTGACAAATCCAAATGGGTGAATCAACTGCTGACATCGTGCGTTATCAATACTCAGTATTGGCAGAAAACAATGCAACTTACACATTTATTGATTACATT

 

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