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CRIMIC CR02773

Gene eIF1A

CG8053

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:18276042..18276043	91A1	FBti0228141	97608

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGCGATTCTGAAACTAAGCTAGTG	TCCGATTGGAGTCCACTTCTGGTA

Images

Construct Design Sequences

sgRNA sequence	TCACCTACCTGAGTTAGCCCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTTTTCAATTTTTTCATGGTAGTACACCTTACGCAAAATTTTGCGTATATCTTTTGCATTTGAATTAGCTATTCAGTTACATGTTTTCAATTGCGAATACAGCCTCCTTTATAGCACTAATTTGGGTGGTCAAACTGCATTCCATGAAATACCATACGTCCGCAATGGTAGTTCCTCCTTATCACCTACCTGAGTTAG	CCCTGGTCGTGGTCACGGTCGTCCATATAGAGATTAGATCGTTTCTTCCGAAGGGAATGAGCACATGTCAAATTTTGGATTGTGCTTCACGCCGCAGGAGACGGGCGTCATAAATAAGATTCCATCCAAATCGACTGCGTCAAGATTCGCGGCGTAATTAGTGACGTCTTCGATTTCTAGTAGGTGTCAAGTAAATATAC

 

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