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CRIMIC CR02775

Gene CAHbeta

CG11967

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	3R:8981729..8981730	85C1	FBti0228142	97609

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTTACTTGAATGTCGTGCACCT	GGAATCGGCAGGAATGTTAAGCCA

Images

Construct Design Sequences

sgRNA sequence	CCTTGAACTCATTGACGCATTGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAGAACAAAAATATAAAAAAAAAGGATACCAAATCTTGTTTGTTTAATTAAATTACAACTTGTATAAGAAATTAATTGCCGAAATTTATATTTCTATAGAAAACATGGGCAGAGGCTTCCTCCAAGATATGTTCGTCCGTCAATAGTTTCAATATATGTATTTGAATTTCCCTGTTGTACAGCCTTGAACTCATTGACG	CATTGGCCATTTGCCAAGTATGTCATAACTTTGGGTGGCTATATTCGGTGGCCGATAGCTAGGTATTCTAACATTTAATATTGATAAACTTTATCATTTAGCCCAAGGCCGTCTTCTTCACCTGCATGGACAGTAGAATGATTCCCACCCGGTACACCGACACTCACGTGGGCGATATGTTTGTGGGTGAGTAAATCCAG

 

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