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CRIMIC CR02780

Gene CtsB

CG10992

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p0	X:13779847..13782170	12C4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTTCTACGCCGAAATAGTTAGAAAGC	ATTTCACGCACATTACGCCTCACC

Images

Construct Design Sequences

sgRNA sequence	GTTGAGGATGTACGCGAATCTGG
sgRNA2 sequence

 

left_200bp	right_200bp
ACTTGATTTTGTTCTTTGTTTACTTGAAGTGAACTGAAGACTCAGTTTACGACGCCATAAAAAATTGTATACCACAGATATCGCTAGAAAGCGGCTTAGTATATCATATACAAACATCCATATACACAAAGCTATATGTATATATATCATATACGAACATCTATAGATATACATGCATATATTGTGAATGCACTCCAGAT	TCGCGTACATCCTCAACGATAAGTTTTAGCTGCTACTAACTCATTTCCCAGATATAGTAAGGCAAACAGAAGAAAAAACGATTCTAATATGCACTTATTTTGCAGGGCTGCCGTCCTTACGAGATTTCTCCCTGTGAGCACCATGTGAATGGAACCCGACCACCATGTGCCCATGGCGGTAGAACGCCCAAGTGTTCGCA

 

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