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CRIMIC CR02791

Gene PK1-R

CG9918

Information

Donor	Location	Cytology	FBti	Stock number
int200_PM37_p1	3R:14099832..14103122	88A8

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGCTAACCATTAAGTAAACATGACTTA	CAGAGGAAGAAGCACACCACCA

Images

Construct Design Sequences

sgRNA sequence	CGGGTTGATTAGCCCAGCTTAGG
sgRNA2 sequence

 

left_200bp	right_200bp
ATTAGTAAGAAATTTTGACAAAAACAAAAGTAGAAGACTCCGTTTTTAATAAGGTTTAGTCTTCTCACAAAAGTCTTGCAAATTAAAAATTCTGAAATATGATATCTAAATCTTTCTTTATATGCAATGAAGAACCATTTTAGAAGCTTTACTTCAACTTTTGCTCAGTGTATGCCAACTGTTTGACCATATGCCCTAAG	CTGGGCTAATCAACCCGTCTGCAGCCAATTTACTGCGTTTGTCATTTTGGCACTCTCCATTTATGATGACAATTGCGATTACCAAAAAATATAATCGTTGACTCTTTATCTGTGACTCTCGCTGTGTCTTCCCCATTTCCATTGCCATTCCATTTTCATCCCCACGTCACTGACTTTTACCGCCCCCCCCTCCCCTTTAG

 

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